Journal: Frontiers in Pharmacology

Article Title: Establishment of a human nasal epithelium model of histamine-induced inflammation to assess the activity of fexofenadine as an inverse agonist and its link to clinical benefit

doi: 10.3389/fphar.2024.1393702

Figure Lengend Snippet: Reconstituted human nasal epithelium model (MucilAir™) to study the effect of HIS and FEX. CBF, cilia beating frequency; ELISA, enzyme-linked immunosorbent assay; LDH, lactate dehydrogenase; MCC, mucociliary clearance; RT-qPCR, quantitative reverse transcription–polymerase chain reaction; TEER, trans-epithelial electrical resistance.

Article Snippet: The basolateral medium was collected and measured using a Cytotoxicity Lactate Dehydrogenase (LDH) Assay Kit, following the manufacturer’s instructions (WST Dojindo, CK12-20).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Reverse Transcription, Polymerase Chain Reaction

Journal: Stem Cell Reviews

Article Title: Human Menstrual Blood-Derived Stromal Cells Promote Recovery of Premature Ovarian Insufficiency Via Regulating the ECM-Dependent FAK/AKT Signaling

doi: 10.1007/s12015-018-9867-0

Figure Lengend Snippet: Physiological changes to evaluate ovarian status. a Serum hormone levels assessed by ELISA kits in each group for measuring FSH (left), E2 (middle) and AMH (right), n = 6 (data were represented as mean ± SEM, * P < 0.05, ** P < 0.01). b Representative images of parturition in each group after mating with male mice. c Records of live births after CTX injection followed by MenSCs transplantation, n = 6 (data were represented as mean ± SEM, ** P < 0.01). d Apoptosis evaluation using Hoechst kit. Yellow arrows pointed to apoptotic cells with fragmented or condensed nuclei of apoptotic cells. Original magnification, ×100. e Apoptosis evaluation performed by TUNEL assay. White arrows pointed to FITC-labeled apoptotic cells. Original magnification, ×100. f Relative mRNA expression by q-RT PCR analysis for AMH, DDX4 and VEGFA controlled to GAPDH with fold change measured by 2 -ΔΔCT , n = 6. g Relative expression at protein levels of AMH, DDX4 and VEGFA (upper panel) and quantitative analysis (lower panel)

Article Snippet: TUNEL assay was performed using TUNEL FITC Apoptosis Detection Kit (Vazyme, Nanjing, China).

Techniques: Enzyme-linked Immunosorbent Assay, Injection, Transplantation Assay, TUNEL Assay, Labeling, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: BMC Complementary and Alternative Medicine

Article Title: Protective effect of topical Cordia verbenacea in a rat periodontitis model: immune-inflammatory, antibacterial and morphometric assays

doi: 10.1186/1472-6882-12-224

Figure Lengend Snippet: Panel illustrating data for morphometric, immune-inflammatory, and antibacterial assays. A and B ) Representative photographs illustrating the morphometric findings of C. verbenacea essential oil group ( A ) and non-treatment group (B). C ) Means and standard error of the mean (SEM) of alveolar bone loss (mm) for ligated and unligated teeth in both groups. Significant difference when compared to ligated teeth (*). Significant difference when compared to NT group (†) (student t test, p < 0.05). D ) Means and SEM of IL-1α and IL-10 concentration (pg/ml) assayed by ELISA. Significant difference when compared to NT group (‡) by student t test, p < 0.05. E ) Frequency (%) of detection of periodontal pathogens in both groups. Significant difference when compared to NT group (**) by Fisher’s exact test, p < 0.05. F ) Representative reverse transcription–polymerase chain reaction products illustrating periodontal pathogens observed in one animal of the C. verbenacea essential oil group.

Article Snippet: The DNA was then extracted from the subgingival biofilm, as previously described [ ] The PCR amplification was performed using 5uL sample added to 45uL reaction buffer containing 1.5 mM MgSO 4 , 200 uM deoxynucleotide triphosphate, 2 uM of each primer, and 2U of Taq polymerase enzyme (OneTaq Polymerase, New England Biolabs, Ipswich, MA, USA).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Polymerase Chain Reaction